Informatics Publishing Limited

Srinivasa Rao Narala ¹, K. Saraswathi ²

Affiliations
1 Department of Chemistry, R.V.R. & J.C. College of Engineering, Guntur, A.P.,, IN
2 Department of Chemistry, S.V. University, Tirupathi, A.P.,, IN

Abstract

A simple, rapid and sensitive reverse phase high performance liquid chromatographic method has been developed for the determination of Mesalamine in bulk drug and pharmaceutical dosage forms. It was carried out using a mobile phase of methanol and 0.025 M potassium dihydrogen phosphate buffer (65:35 v/v) on a kromosil C18 column (250 X 4.6 mm, 5μm) in an isocratic mode at a flow rate of 1.0 ml/min. with detection at 250 nm using a UV detector. The linearity was obtained in the concentration range of 20 –100 μg/ml. The developed method was found to be accurate for the determination of Mesalamine in tablet dosage forms.

Keywords

Mesalamine, RP-HPLC, Method development, Validation, Recovery experiments.

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